Steve Firth <%steve%@ > wrote in message
news: %%steve%@ ...
> Paul Nutteing (valid email address in post script )
>
>
> > "Peer reviewed" on the site for (mainly) serving
> > forensic scientists
> > /group/forensic-science/
>
> Who all told you that you are an idiot and that you don't know what you
> are talking about.
>
> And making posts to a web forum is no moe "peer review" than your
> posting here.
The only one concerning specifics rather
than sentiments I can think of was this one -:
This is a reply assessment from one of the serving
forensic scientists concerning the anomaly
in the CMJ report , referred to earlier in this
thread.
/2003/44/3/
"Let me break the unbearable "cover-up" silence.
First, have you ever heard of stutter? The vWA loci commonly
experiences this artifact of amplification. Hence what you consider
a 14 allele is probably a stutter peak. Notice I said probably. I
would not be so foolish as to make any kind of definite assertion
(such as yourself) based on the limited data presented in said
article. Without the raw data for analysis no reasonable conclusion
can be drawn from the Figure 1B plot.
Second, let's assume your assumption of a 14 allele at vWA is
correct for the moment. The presence of the extra allele in a mixed
stain is not exculpatory. This is elementary. Again, assuming the
14 allel was a legitimate peak, its presence would only indicate a
possible 3rd contibutor to the stain. Extra alleles do not exclude
contibutors from a mixed stain.
Sorry to destroy the shooter on the grassy knoll... no conspiracy
here! "
He interpreted it as stutter which did not fit
the case in point causing more heated discussion -
the real answer only emerged when someone contacted
the author - the anomaly was bleed-through aka
pull-up, not stutter.
Here is the response from the paper's author...
"Dear mr _
if you look carefully the electropherogram of AmpFlSTR SGM Plus, you
will see no extra peak at vWA locus in Figure 1 plot A, this extra
peak you mention ( plot B) is result of pull-up from the other
color in yellow because the concentration of DNA used in PCR was to
high (RFU is between 4000-6000, but recommendation is 1500 to 2000)."
ps
What they aren't telling you about DNA profiles
and what Special Branch don't want you to know.
/
or nutteingd in a search engine.